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Abstract

Lipoproteins, isolated from ostrich egg yolk (LPFo), provide excellent protection for boar spermatozoa against cryo-induced damage. The present study was performed to investigate the effects of LPFo on the freezability and fertilizing capacity of frozen-thawed (FT) boar semen after post-cervical artificial inseminations (post-CAIs). Semen, collected from 7 Polish Large White (PLW) and 4 Polish Landrace (PLR), was frozen in an extender containing LPFo. Post-CAIs were performed in 38 multiparous sows, using a catheter-cannula kit. Sows were inseminated 2Ă— within one oestrus, and fertility parameters were recorded after farrowing. Neither boar (within breed) nor breed affected the quality of the pre-freeze (PF) semen, such as total motility (TMOT), mitochondria membrane potential (MMP), plasma membrane integrity (PMI), osmotic resistance test (ORT) and DNA fragmentation. Differences in the freezability of boar semen were observed among the boars, whereas there were no marked breed effects. Post-thaw TMOT markedly declined over storage time in most of the boars, particularly at 60 min after thawing. Inseminations of post-weaned oestrus sows resulted in pregnancy and farrowing rates of 84.2% and 81.6%, respectively. Neither the mean number of piglets born (NB) nor the mean number of piglets born alive (NBA) was affected by boar or breed. The total number of piglets born was 365, resulting in 11.8 NB piglets, whereas the total number of piglets born alive was 353, with 11.4 NBA piglets per litter. The findings of this study reaffirm the variations in the freezability of boar semen. In this study the supplementation of ostrich egg yolk lipoproteins to the freezing extender of boar semen produced high proportions of functionally viable FT spermatozoa that were capable of providing acceptable fertility results after post-CAIs in multiparous sows.
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Abstract

The aim of this study was to measure the NO level in boar semen held in a liquid state and to determine its putative relation to spermatozoa motility, plasma membrane integrity, mitochondrial membrane potential and ATP content. Generally, the percentage of spermatozoa which generated nitric oxide gradually increased, while NO level in the surrounding medium declined during the liquid preservation. NO generation in semen preserved in BTS was higher as compared to those in Androhep®Plus. We demonstrated the positive correlation between the NO level in fresh spermatozoa and their quality. We also showed negative correlation between nitric oxide level in spermatozoa preserved in BTS and sperm cells motility as well as plasma membrane integrity. Results obtained in this study confirm that NO may affect sperm physiology in a dualistic manner.
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