Within this article the method of delimitation of the revitalization area within the realities of the current Polish legislation was described. This method was presented on the example of the City of Wejherowo – a medium-sized municipality, being part of the Gdańsk Metropolitan Area. It includes both spatial and socio-economic analyses allowing selection of the areas suff ering from various types of degradation. In result, this method allows indicating the areas demanding the complex intervention, which – on the basis of the comprehensive analysis – shall be subject of further planning for urban regeneration.
This paper includes a description of both the mode of development and the final structure and contents of the Municipal Revitalization Program, which was developed for the City of Starogard Gdański. All key parts of this program were described as well as methods used for planning the diverse activities associated with revitalization of the municipality. These were based on both actual legislation – of both national and regional importnace – as well as on Author’s own experience in this matter. In result, the article can serve as the example of one of the possible ways of developing the complex program of urban transformation for the medium-sized municipality.
To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies, but a system in which SE or ORG can be induced alternatively in the same type of explant has not been proposed. Immature zygotic embryos (IZEs) of Arabidopsis provide the only explants with embryogenic potential, and have been recommended for studying mechanisms of SE induced in vitro. This study was aimed at defining culture conditions promoting induction of alternative morphogenic pathways: shoot ORG in IZE explants. The established protocol involves pretreatment of IZE explants with liquid auxin-rich callus induction (CIM) medium, followed by subculture on solid cytokinin-rich shoot induction medium (SIM). The method enables efficient shoot induction in Columbia (Col-0) and Wassilewskija (Ws), genotypes commonly used in molecular studies. During 3 weeks of culture up to 90% of Col-0 and 70% of Ws explants regenerated shoots via an indirect morphogenic pathway. We analyzed the qRT-PCR expression patterns of the LEC (LEC1, LEC2 and FUS3) genes, the key regulators of Arabidopsis embryogenesis, in the IZE explants induced to promote shoot ORG. The sharp decline of LEC expression on SIM medium confirmed that culture of Arabidopsis IZE explants enables experimental manipulation of the morphogenic response of somatic cells. A scheme illustrating various in vitro morphogenic responses of IZEs in relation to hormonal treatment is presented.