The primary evaluation of the economic losses caused by water pollution in Shanghai in the year 2009 is made by classification approach in order to provide basis for decision of the relative water management policy. The result shows that the portion of water pollution losses in GDP of Shanghai was 2.7%, which was still lower than the average level of whole China despite of the local high population density and the scale of industry, suggesting to some extent the continuous attention in water protection paid by Shanghai government.
In this study, medium-carbon steel was subjected to warm deformation experiments on a Gleeble 3500 thermosimulator machine at temperatures of 550°C and 650°C and strain rates of 0.001 s–1 to 1 s–1. The warm deformation behavior of martensite and the effects of strain rate on the microstructure of ultrafine grained medium-carbon steel were investigated. The precipitation behavior of Fe3C during deformation was analyzed and the results showed that recrystallization occurred at a low strain rate. The average ultrafine ferrite grains of 500 ± 58 nm were fabricated at 550°C and a strain rate of 0.001 s–1. In addition, the size of Fe3C particles in the ferrite grains did not show any apparent change, while that of the Fe3C particles at the grain boundaries was mainly affected by the deformation temperature. The size of Fe3C particles increased with the increasing deformation temperature, while the strain rate had no significant effect on Fe3C particles. Moreover, the grain size of recrystallized ferrite decreased with an increase in the strain rate. The effects of the strain rate on the grain size of recrystallized ferrite depended on the deformation temperature and the strain rate had a prominent effect on the grain size at 550°C deformation temperature. Finally, the deformation resistance apparently decreased at 550°C and strain rate of 1 s–1 due to the maximum adiabatic heating in the material.
In order to compare the pathogenicity of different Tembusu virus (TMUV) strains from geese, ducks and chickens, 56 5-day-old Cherry Valley ducklings which were divided into 7 groups and infected intramuscularly with 7´105 PFU/ml per duck of six challenge virus stocks. The clinical signs, weight gain, mortality, macroscopic and microscopic lesions, virus loads in sera of 1, 3, 5, 7, 11 and 14 dpi and serum antibody titers were examined. The results showed that these viruses could make the young ducks sick, but the clinical signs differed with the different species-original strains. All the experimental groups lose markedly in weight gain compared to the control, but there were no obvious distinctions in weight gains, as well as macroscopic and microscopic lesions of dead ducks between the infected groups. However, the groups of waterfowl-derived strains (from geese and ducks) showed more serious clinical signs and higher relative expressions of virus loads in sera than those from chicken-derived. The mortality of waterfowl groups was 37.5%, and the greatest mortality of chicken groups was 12.5%. The serum antibodies of the geese-species group JS804 appeared earlier and were higher in the titers than others. Taken toghter, the pathogenicity of waterfowl-derived TMUV was more serious than chicken-derived TMUV and JS804 could be chosen as one TMUV vaccine strain to protect from the infection.
The field of mechanical manufacturing is becoming more and more demanding on machining accuracy. It is essential to monitor and compensate the deformation of structural parts of a heavy-duty machine tool. The deformation of the base of a heavy-duty machine tool is an important factor that affects machining accuracy. The base is statically indeterminate and complex in load. It is difficult to reconstruct deformation by traditional methods. A reconstruction algorithm for determining bending deformation of the base of a heavy-duty machine tool using inverse Finite Element Method (iFEM) is presented. The base is equivalent to a multi-span beam which is divided into beam elements with support points as nodes. The deflection polynomial order of each element is analysed. According to the boundary conditions, the deformation compatibility conditions and the strain data measured by Fiber Bragg Grating (FBG), the deflection polynomial coefficients of a beam element are determined. Using the coordinate transformation, the deflection equation of the base is obtained. Both numerical verification and experiment were carried out. The deflection obtained by the reconstruction algorithm using iFEM and the actual deflection measured by laser displacement sensors were compared. The accuracy of the reconstruction algorithm is verified.
Ludwigite is the main available boron-bearing resource in China. In order to enrich the theory system and optimize its utilization processes, this paper study the mechanism and kinetics on non-isothermal decomposition of ludwigite in inert atmosphere by means of thermal analysis. Results show that, the decomposition of serpentine and szajbelyite is the main cause of mass loss in the process. At the end of decomposition, hortonolite and ludwigite are the two main phases in the sample. The average E value of structural water decomposition is 277.97 kJ/mol based on FWO method (277.17 kJ/mol based on KAS method). The results is proved to be accurate and reliable. The mechanism model function of structural water decomposition is confirmed by Satava method and Popescu method. The form of the most probable model function is G(α) = (1 – α)–1 – 1 (integral form) and f (α) = (1 – α)2 (differential form), and its mechanism is chemical reaction. This is verified by the criterion based on activation energy of model-free kinetics analysis.
One of the most critical aspects of mine design is to determine the optimum cut-off grade. Despite Lane’s theory, which aims to optimize the cut-off grade by maximizing the net present value (NPV), which is now an accepted principle used in open pit planning studies, it is less developed and applied in optimizing the cut-off grade for underground polymetallic mines than open pit mines, as optimization in underground polymetallic mines is more difficult. Since there is a similar potential for optimization between open pit mines and underground mines, this paper extends the utilization of Lane’s theory and proposes an optimization model of the cut-off grade applied to combined mining-mineral processing in underground mines with multi-metals. With the help of 3D visualization model of deposits and using the equivalent factors, the objective function is expressed as one variable function of the cut-off grade. Then, the curves of increment in present value versus the cut-off grade concerning different constraints of production capacities are constructed respectively, and the reasonable cut-off grade corresponding to each constraint is calculated by using the golden section search method. The defined criterion for the global optimization of the cut-off grade is determined by maximizing the overall marginal economics. An underground polymetallic copper deposit in Tibet is taken as an example to validate the proposed model in the case study. The results show that the overall optimum equivalent cut-off grade, 0.28%, improves NPV by RMB 170.2 million in comparison with the cut-off grade policy currently used. Thus, the application of the optimization model is conducive to achieving more satisfactory economic benefits under the premise of the rational utilization of mineral resources.
In a PV-dominant DC microgrid, the traditional energy distribution method based on the droop control method has problems such as output voltage drop, insufficient power distribution accuracy, etc. Meanwhile, different battery energy storage units usually have different parameters when the system is running. Therefore, this paper proposes an improved control method that introduces a reference current correction factor, and a weighted calculation method for load power distribution based on the parameters of battery energy storage units is proposed to achieve weighted allocation of load power. In addition, considering the variation of bus voltage at the time of load mutation, voltage secondary control is added to realize dynamic adjustment of DC bus voltage fluctuation. The proposed method can achieve balance and stable operation of energy storage units. The simulation results verified the effectiveness and stability of the proposed control strategy.
The extracellular polymeric substance (EPS) produced from Rhizobium radiobacter F2, designated as EPSF2, was investigated as a biosorbent for the removal of Pb(II) and Zn(II) from aqueous solution. The optimum biosorption pH values were 5.0 for Pb(II) and 6.0 for Zn(II). Kinetics study revealed that the biosorption followed pseudo-first-order model well, and the equilibrium data fit the Langmuir model better. The adsorbed metal ions could be effectively desorbed by HCl. Desrobed EPSF2 regained 80% of the initial biosorption capacity after five cycles of biosorption-desorption-elution. These results demonstrated that EPSF2 could be a promising alternative for Pb(II) and Zn(II) removal from aqueous solution.
MDAP-2 is a new antibacterial peptide with a unique structure that was isolated from house- flies. However, its biological characteristics and antibacterial mechanisms against bacteria are still poorly understood. To study the biological characteristics, antibacterial activity, hemolytic activi- ty, cytotoxicity to mammalian cells, and the secondary structure of MDAP-2 were detected; the results showed that MDAP-2 displayed high antibacterial activity against all of the tested Gram-negative bacteria. MDAP-2 had lower hemolytic activity to rabbit red blood cells; only 3.4% hemolytic activity was observed at a concentration of 800μg/ml. MDAP-2 also had lower cytotoxicity to mammalian cells; IC50 values for HEK-293 cells, VERO cells, and IPEC-J2 cells were greater than 1000 μg/ml. The circular dichroism (CD) spectra showed that the peptide most- ly has α-helical properties and some β-fold structure in water and in membrane-like conditions. MDAP-2 is therefore a promising antibacterial agent against Gram-negative bacteria. To deter- mine the antibacterial mechanism(s) of action, fluorescent probes, flow cytometry, and transmis- sion electron microscopy (TEM) were used to study the effects of MDAP-2 on membrane perme- ability, polarization ability, and integrity of Gram-negative bacteria. The results indicated that the peptide caused membrane depolarization, increased membrane permeability, and destroyed membrane integrity. In conclusion, MDAP-2 is a broad-spectrum, lower hemolytic activity, and lower cytotoxicity antibacterial peptide, which is mainly effective on Gram-negative bacteria. It exerts its antimicrobial effects by causing bacterial cytoplasm membrane depolarization, increas- ing cell membrane permeability and disturbing the membrane integrity of Gram-negative bacte- ria. MDAP-2 may offer a new strategy to for defense against Gram-negative bacteria.
In vitro embryogenic callus is a critical factor for genetic transformation of rice, especially for indica varieties. In this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), and callus browning, and we studied the effect of exogenous Put on callus regeneration and on the content of endogenous polyamines. In addition, the expression levels of arginine decarboxylase gene (Adcl) and S-adenosylmethionine decarboxylase gene (Samdc) in embryogenic callus were studied by quantitative Real-time PCR analysis. The results showed that the contents of endogenous Put and Spd in the browning callus were significantly lower than those in normal callus. Exogenous Put could effectively improve the growing state of callus of indica rice and enhance the development of embryogenic callus. The content of endogenous polyamines in embryogenic callus, especially Spd and Spm, was increased after addition of exogenous Put. Additionally, exogenous Put also had an obvious impact on the expression levels of Adcl but partial effect on the expression levels of Samdc gene. This study could increase the knowledge of both embryogenic callus induction and polyamine catabolism in callus in indica rice.
Isothermal hot compression experiments were carried out using the Gleeble-1500D thermal mechanical simulator. The flow stress of the Cu-1%Zr and Cu-1%Zr-0.15%Y alloys was studied at hot deformation temperature of 550°C, 650°C, 750°C, 850°C, 900°C and the strain rate of 0.001 s–1, 0.01 s–1, 0.1 s–1, 1 s–1, 10 s–1. Hot deformation activation energy and constitutive equations for two kinds of alloys with and without yttrium addition were obtained by correlating the flow stress, strain rate and deformation temperature. The reasons for the change of hot deformation activation energy of the two alloys were analyzed. Dynamic recrystallization microstructure evolution for the two kinds of alloys during hot compression deformation was analyzed by optical and transmission electron microscopy. Cu-1%Zr and Cu-1%Zr-0.15%Y alloys exhibit similar behavior of hot compression deformation. Typical dynamic recovery occurs during the 550-750°C deformation temperature, while dynamic recrystallization (DRX) occurs during the 850-900°C deformation temperature. High Zr content and the addition of Y significantly improved Cu-1%Zr alloy hot deformation activation energy. Compared with hot deformation activation energy of pure copper, hot deformation activation energy of the Cu-1%Zr and Cu-1%Zr-0.15%Y alloys is increased by 54% and 81%, respectively. Compared with hot deformation activation energy of the Cu-1%Zr alloy, it increased by 18% with the addition of Y. The addition of yttrium refines grain, advances the dynamic recrystallization critical strain point and improves dynamic recrystallization.
Geomechnical model testing has been widely applied as a kind of research technique in underground engineering problems. However, during the practical application process, due to the influence of many factors, the desired results cannot be obtained. In order to solve this problem, based on the measurement requirements of the model test, combined with FBG(Fiber Bragg Grating) sensor technology and traditional measurement methods, an FBG monitoring system, Micro-multi-point displacement test system, resistance strain test system and surrounding rock pressure monitoring system are developed. Applying the systems to a model test of the tunnel construction process, the displacement in advance laws of tunnel face, radial displacement distribution laws and surrounding rock pressure laws are obtained. Test results show that a multivariate information monitoring system has the advantage of high precision, stability and strong anti-jamming capability. It lays a solid foundation for the real-time data monitoring of the tunnel construction process model test.