We used artificial hybridization to study the crossability of the noble fir (Abies procera) with Manchurian fir (A. holophylla) and Caucasian fir (A. nordmanniana), and found compatibility between A. procera of North American origin and the Asian species A. holophylla as evidenced by the 14% fraction of filled seeds obtained in A. procera × A. holophylla crossing. Crossing of A. procera with the Mediterranean species A. nordmanniana failed completely, producing only empty seeds.
Abstract Gene flow among individual trees of Pinus sylvestris and P. mugo putative hybrid swarms in Slovakia was fol-lowed at four localities using the species-diagnostic cpDNA trnV-trnH/Hinf I restriction profile. Variable proportions of P. sylvestris and P. mugo haplotypes were revealed among the sampled localities. Low between-habitus consistency of the trees and their cpDNA haplotypes indicates the hybrid nature of the swarms. Molecular analy-sis based on mutual comparison of the haplotypes of a given tree and its embryos suggests direct and recipro-cal hybridization between trees of the P. sylvestris and P. mugo haplotypes. Besides conspecific embryos resulting from hybridization of trees with the same haplotype (P. sylvestris × P. sylvestris and P. mugo × P. mugo), hybrid embryos of P. sylvestris × P. mugo (8.03%) and P. mugo × P. sylvestris (11.50%) were also detected in open-pollinated offspring. The results are discussed from the standpoint of primary and introgressive hybridization between the parental species.