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Abstract

The aim of this paper is to present methods of digitally synthesising the sound generated by vibroacoustic systems with distributed parameters. A general algorithm was developed to synthesise the sounds of selected musical instruments with an axisymmetrical shape and impact excitation, i.e., Tibetan bowls and bells. A coupled mechanical-acoustic field described by partial differential equations was discretized by using the Finite Element Method (FEM) implemented in the ANSYS package. The presented synthesis method is original due to the fact that the determination of the system response in the time domain to the pulse (impact) excitation is based on the numerical calculation of the convolution of the forcing function and impulse response of the system. This was calculated as an inverse Fourier transform of the system’s spectral transfer function. The synthesiser allows for obtaining a sound signal with the assumed, expected parameters by tuning the resonance frequencies which exist in the spectrum of the generated sound. This is accomplished, basing on the Design of Experiment (DOE) theory, by creating a meta-model which contains information on its response surfaces regarding the influence of the design parameters. The synthesis resulted in a sound pressure signal in selected points in space surrounding the instrument which is consistent with the signal generated by the actual instruments, and the results obtained can improve them.
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Abstract

Aeromonas hydrophila is a valuable indicator of the quality of water polluted by sewage and pathogens that pose a risk for humans and cold-blooded animals, including fi sh. The main aim of this research was to evaluate anthropogenic pollution of river water based on genetic diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR (ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A. hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi random and rep-BOX). None of the analyzed electrophoretic patterns was identical, implying that there were many sources of strain transmission. The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains, and drug resistance patterns for tetracycline, enrofl oxacin and erythromycin were determined. The most diverse A. hydrophila strains isolated from river water were susceptible to enrofl oxacine (HS = 0.27), whereas less diverse strains were susceptible to erythromycin (HS = 0.24). The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus) in the examined strains (resistant to three analyzed drugs) indicates that intensive fi sh cultivation affects the microbiological quality of river water.
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