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Number of results: 16
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Abstract

The aim of the study was to develop new laparoscopic technique for repeated recovery of sheep oocytes. Oocytes were aspirated with specifically designed catheter. It allowed to recover oocytes without ovary damage and to preserve very good quality of recovered oocytes. Fifteen ewes were oocytes donors. Oocytes were collected: one time (group I, n=15), two times (group II, n=15), three times (group III, n=10), four times (group IV, n=5). The endoscope was inserted into the abdominal cavity. Two trockars for putting the manipulators were inserted 15 cm cranial from the udder. Oocytes were collected by aspiration of the follicular fluid from the ovarian follicles. The observed clinical complications were: ovary bleeding and cicatrix at place of needle insertion, the fragmentary adhesion of infundibulum and ovary, adhesions of omentum and peri- toneum near the place where the grasping forceps were inserted and adhesion of ovary and uterus. Ovarian follicles (n=204) were aspirated, 130 (63.8%) oocytes were obtained. Out of 130 obtained oocytes, 112 were qualified for in vitro maturation. The remaining 18 oocytes (13.8%) were rejected due to cytoplasmic changes. The proposed technique allows for the collecting oocytes of good quality that can be used for IMV/IVF techniques and cloning.
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Abstract

3D printing in FDM (Fused Deposition Modelling) technology is commonly used, mainly in the preparation of prototypes, but also for the production of ready-made elements. Objects printed using the FDM method have characteristic, adverse surface features related to the limitations of this technology. That is why surface treatment of 3D prints becomes crucial. One of the method is metal plating of elements. The most frequently used material in FDM technology is PLA (polylactic acid) and ABS (acrylonitrile butadiene styrene). Study of surface parameters determination for ABS prints after galvanic copper plating is presented in this paper. For this purpose, samples printed with ABS were smoothed in acetone vapour. Most favorable parameters of the surface were obtained for samples that had contact with acetone vapour for 60 minutes. Ultimately, surface analysis of samples after graphite coating and subjected to copper plating was performed. It was found that surface parameters are close to results obtained with traditional methods of metal processing.
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