Considering concrete nonlinearity, the wave height limit between small and large amplitude sloshing is defined based on the Bernoulli equation. Based on Navier-Stokes equations, the mathematical model of large amplitude sloshing is established for a Concrete Rectangle Liquid-Storage Structure (CRLSS). The results show that the seismic response of a CRLSS increases with the increase of seismic intensity. Under different seismic fortification intensities, the change in trend of wave height, wallboard displacement, and stress are the same, but the amplitudes are not. The areas of stress concentration appear mainly at the connections between the wallboards, and the connections between the wallboard and the bottom.
Vaccination is a common routine for prevention and control of human and animal diseases by inducing antibody responses and cell-mediated immunity in the body. Through vaccinations, smallpox and some other diseases have been eradicated in the past few years. The use of a patho- gen itself or a subunit domain of a protein antigen as immunogens lays the basis for traditional vaccine development. But there are more and more newly emerged pathogens which have expe- rienced antigenic drift or shift under antibody selective pressures, rendering vaccine-induced im- munity ineffective. In addition, vaccine development has been hampered due to problems includ- ing difficulties in isolation and culture of certain pathogens and the antibody-dependent enhancement of viral infection (ADE). How to induce strong antibody responses, especially neu- tralizing antibody responses, and robust cell-mediated immune responses is tricky. Here we re- view the progress in vaccine development from traditional vaccine design to reverse vaccinology and structural vaccinology and present with some helpful perspectives on developing novel vac- cines.
Recycling construction and demolition waste not only reduces project costs; and saves natural resources, but also solves the environmental threat caused by construction waste disposal. In this paper, C25 waste road concrete is used as an experimental material, the uniaxial compression strength and tensile splitting strength of C25 RAC whose coarse aggregate replacement rate is 0%, 25%, 50%, 75%, and 100% are tested under the condition that the water-to-cement ratio is 0.47, 0.55 and 0.61. The results show: (1) the uniaxial compression strength and tensile splitting strength decrease with the increase of RAC; (2) for concrete with the same water-to-cement ratio, when the coarse aggregate replacement rate changes from 0% to 50%, the uniaxial compression strength and tensile splitting strength of RAC changes slightly. When the coarse aggregate replacement rate changes from 50% to 100%, the uniaxial compression strength and tensile splitting strength of RAC decreases rapidly
Heterogeneous nuclear ribonucleoprotein K (hnRNP K), is a multifunctional protein that participates in a variety of regulatory processes of signal transduction and gene expression. To further characterize the significance of hnRNP K in different male germ cells, we investigated the expression profiles of hnRNP K at different developmental stages in pig and rat testes, and conducted a comparative analysis of expression patterns between these two species. In porcine testis development, both the mRNA and protein level of hnRNP K were down-regulated from 3 months to 8 months. However, the expression level of hnRNP K was abundant across the embryonic period in rats, and decreased gradually from 0 day post partum (dpp) to 14 dpp, then increased with the highest level presenting at 90 dpp. Immunolocalization analysis further confirmed the differential expression and localization of hnRNP K protein during testis development in pigs and rats. The results showed that hnRNP K was widely distributed in gonocytes, spermatogonia, sertoli cells and Leydig cells. The dynamic expression profile of hnRNP K may imply its crucial and potential roles in the development of the testis, which will provide a theoretical basis for the future study of molecular mechanism regulation of spermatogenesis.
Sapelovirus A (SV-A) is a positive-sense single-stranded RNA virus which is associated with acute diarrhea, pneumonia and reproductive disorders. The virus capsid is composed of four proteins, and the functions of the structural proteins are unclear. In this study, we expressed SV-A structural protein VP1 and studied its antigenicity and immunogenicity. SDS-PAGE analysis revealed that the target gene was expressed at high levels at 0.6 mM concentration of IPTG for 24 h. The mouse polyclonal antibody against SV-A VP1 protein was produced and reached a high antiserum titer (1: 2,048,000). Immunized mice sera with the recombinant SV-A VP1 protein showed specific recognition of purified VP1 protein by western blot assay and could recognize native SV-A VP1 protein in PK-15 cells infected with SV-A by indirect immunofluorescence assay. The successfully purified recombinant protein was able to preserve its antigenic determinants and the generated mouse anti-SV-A VP1 antibodies could recognize native SV-A, which may have the potential to be used to detect SV-A infection in pigs.