The authors paid particular attention to the problem of antenna impedance measurements in the RFID technique. These measurements have to be realized by using two ports of a vector network analyzer and dedicated passive differential probes. Since the measurement process and estimated parameters depend on the frequency band, operating conditions, type of the system component and antenna designs used, appropriate verification of the impedance parameters on the basis of properly conducted experiments is a crucial stage in the antenna synthesis of transponders and read/write devices. Accordingly, a systematized procedure of impedance measurements is proposed. It can be easily implemented by designers preparing antennas for different kinds of RFID applications. The essence of indirect measurements of the differential impedance parameters is discussed in details. The experimental verification has been made on the basis of a few representative examples.