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Abstract

This research was conducted to investigate the natural, quantitative composition of the most common Fusarium species directly in fields of northeastern Poland. The concentration of Fusarium spp. and grain quality traits (yield, 1,000 kernel weight, test weight, grain moisture, ergosterol content, protein content, gluten content and starch content) were compared in four wheat varieties (Mandaryna, Struna, Kandela and Arabella). Obtained results indicated a relation between grain moisture, test weight, ergosterol content, yield and fungi concentration. Protein, starch and gluten content was similar in all wheat varieties. Fusarium culmorum was the most common pathogen in Mandaryna and Struna and F. graminearum in Kandela and Arabella. Fusarium avenaceum and F. poae occurred in low amounts in all wheat varieties except Mandaryna. Fusarium oxysporum was found in comparable concentrations in Struna, Kandela and Arabella. Struna despite medium Fusarium spp. colonization possessed the most desirable grain quality compared to other varieties. We carried out real-time PCR detection of Fusarium spp. which is an efficient, cost effective and time saving method in evaluating the development of fungal diseases which are not visible in standard observations.
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Abstract

The study was performed on 21 H-F calves divided into 3 groups of 7 animals each. The first group was composed of calves whose mothers did not receive an injection of Se preparation. The second and third groups consisted of calves whose mothers were administered injections of Se and vitamin E in a single dose of 10 ml and 30 ml, 10 days before the expected parturition date. 24 hours after birth, blood samples were collected from all calves to determine Se, Fe and IgG concentrations and the activity of GSH-Px and GGT. The results of the study indicate that the administration of a single-dose Se supplement to cows in late pregnancy increases Se concentration in calves and promotes passive transfer of immunity from the mother to offspring.
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Abstract

Cytological evaluation of bone marrow smears stained by May-Grünwald Giemsa method was performed. The smears came from 20 fallow deer (Dama dama) 3 days old divided into 2 groups each consisting of 10 animals. The experimental group (E) received intramuscularly selenium and vitamin E at a dose of 3.0 ml (tocopherol acetate – 50 mg, sodium selenite – 0.5 mg, solvent - 1 ml) in the 3rd day of age. The control group (C) did not receive any supplementation or placebo. For hematological analyzes blood was collected three times: on 0, 15th and 25th day of the experiment. Serum concentration of selenium and vitamin E was determined using high perfor- mance liquid chromatography and glutathione peroxidase activity (GSH-Px) by kinetic method. On the 15th day after supplementation, a statistically significant increase in the percentage of erythroblastic cell line was observed in bone marrow smears. At that time, the increase in GSH-Px activity in the E group was also observed, reaching the value of 165.3 U/gHb, which was statisti- cally significant. The percentage of proerythroblasts (8.23% in group E and 5.02% in group C) differed significantly between groups at the 25th day after supplementation. This study revealed that supplementation of selenium and vitamin E resulted in an increase in the number of erythro- cytes to an average of 13.5 (˟ 10¹²/l) in the experimental group on 25th day with a significant increase in hemoglobin to 193 g/l in the experimental group.
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