The locally resonant sonic material (LRSM) is an artificial metamaterial that can block underwater sound. The low-frequency insulation performance of LRSM can be enhanced by coupling local resonance and Bragg scattering effects. However, such method is hard to be experimentally proven as the best optimizing method. Hence, this paper proposes a statistical optimization method, which first finds a group of optimal solutions of an object function by utilizing genetic algorithm multiple times, and then analyzes the distribution of the fitness and the Euclidean distance of the obtained solutions, in order to verify whether the result is the global optimum. By using this method, we obtain the global optimal solution of the low-frequency insulation of LRSM. By varying parameters of the optimum, it can be found that the optimized insulation performance of the LRSM is contributed by the coupling of local resonance with Bragg scattering effect, as well as a distinct impedance mismatch between the matrix of LRSM and the surrounding water. This indicates coupling different effects with impedance mismatches is the best method to enhance the low-frequency insulation performance of LRSM.
A mixed pseudo-orthogonal frequency coding (Mixed-POFC) structure is proposed as a new spreadspectrum technique in this paper, which employs frequency and time diversity to enhance tag properties and balances the spectrum utilization and code diversity. The coding method of SAW RFID tags in this paper uses Mixed-POFC with multi-track chip arrangements. The cross-correlation and auto correlation of Mixed-POFC and POFC are calculated to demonstrate the reduced overlap between the adjacent center frequencies with the Mixed-POFC method. The center frequency of the IDT and Bragg reflectors is calculated by a coupling of modes (COM) module. The combination of the calculation results of the Bragg reflectors shows that compared with a 7-chip POFC, the coding number of a 7-chip Mixed-POFC is increased from 120 to 144 with the same fractional bandwidth of 12%. To demonstrate the validity of Mixed-POFC, finite element analysis (FEA) technology is used to analyze the frequency characteristics of Mixed-POFC chips. The maximum error between designed frequencies and simulation frequencies is only 1.7%, which verifies that the Mixed-POFC method is feasible.
In this study, medium-carbon steel was subjected to warm deformation experiments on a Gleeble 3500 thermosimulator machine at temperatures of 550°C and 650°C and strain rates of 0.001 s–1 to 1 s–1. The warm deformation behavior of martensite and the effects of strain rate on the microstructure of ultrafine grained medium-carbon steel were investigated. The precipitation behavior of Fe3C during deformation was analyzed and the results showed that recrystallization occurred at a low strain rate. The average ultrafine ferrite grains of 500 ± 58 nm were fabricated at 550°C and a strain rate of 0.001 s–1. In addition, the size of Fe3C particles in the ferrite grains did not show any apparent change, while that of the Fe3C particles at the grain boundaries was mainly affected by the deformation temperature. The size of Fe3C particles increased with the increasing deformation temperature, while the strain rate had no significant effect on Fe3C particles. Moreover, the grain size of recrystallized ferrite decreased with an increase in the strain rate. The effects of the strain rate on the grain size of recrystallized ferrite depended on the deformation temperature and the strain rate had a prominent effect on the grain size at 550°C deformation temperature. Finally, the deformation resistance apparently decreased at 550°C and strain rate of 1 s–1 due to the maximum adiabatic heating in the material.
The heavy metal release experiments were conducted in the laboratory to examine the effects of 3 factors - pH, dissolved oxygen (DO), and temperature on the metal release from sediments taken from the Huangpu River. The metal concentrations in the dry sediments ranged from 0.030 to 0.296 mg g-1 for Cr, 0.021 to 0.097 mg g-1 for Ni, 0.014 to 0.219 mg g-1 for Cu, 0.035 mg to 0.521 mg g-1 for Zn, 0.0002 to 0.001 mg g-1 for Cd and 0.023 to 0.089 mg g-1 for Pb. Most of the metals found in the sediments were in the form of residual fraction, the exchangeable fraction consisted of only a small portion of total metals. The average dissolved metal concentrations in the overlying water during the 13-day period under different conditions were ranging from 0.82 to 1.93 μg L-1 for Cr, 1.08 to 4.19 μg L-1 for Ni, 40.79 to 82.28 μg L-1 for Cu, 20.30 to 29.96 μg L-1 for Zn, 1.57 to 4.07 μg L-1 for Cd, and 22.26 to 75.50 μg L-1 for Pb, respectively. Statistical interpretation of the data indicated that pH (7, 8, 9), dissolved oxygen DO (1.0 and 5.0 mg L-1) and temperature (4, 16, 25°C) had no significant effects on the heavy metal release under the studied conditions. Cu and Pb had the highest release flux, while Cd, Pb and Cu had higher mobility. The main factors controlling the metals release might be the inherent characters of metals and sediments.
The technology for gob-side entry retaining in steep coal seams is still in the development stage. The analysis results of the caving structure of main roof, low influence of gateway’s stability because of long filling distance and weak dynamic effect of the gateway, and the low stress redistribution environment indicate that using this technology in steep coal seams has significant advantages. Moreover, to reinforce the waste rock and the soft floor and to better guard against the impact of the waste rock during natural filling, a rock blocking device and grouting reinforcement method were invented, and theoretical calculations result show that the blocking device has high safety factor. In addition, we also developed a set of hydraulic support devices for use in the strengthening support zone. Furthermore, because the retaining gateway was a systematic project, the selection of the size and shape of the gateway cross section and its support method during the initial driving stage is a key step. Thus, first, a section the size of bottom width and roof height of a new gateway was determined to meet any related requirements. Then, according to the cross sections of 75 statistical gateways and the support technique, it chosen a trapezoidal cross section when the dip of the coal seam is 35° < α ≤ 45°, a special and an inclined arch cross section when 45° < α ≤ 55°. Eventually, a support system of bolts and cables combined with steel mesh and steel belts was provided. The support system used optimized material and improved parameters, can enhanced the self-bearing ability of the surrounding coal and rock masses.
Abstract NH4+ is an important N-source which regulates plant growth and development. However, the underlying mechanism of NH4+ uptake and its-mediated signaling is poorly understood. Here, we performed phosphoproteomic studies using the titanium dioxide (TiO2)-mediated phosphopeptides collection method together with LC-MS analysis. The results indicated that phosphorylation levels of 23 and 43 peptides/proteins involved in diverse aspects, including metabolism, transport and signaling pathway, were decreased and increased respectively after NH4+ treatment in rice roots. Among 23 proteins detected, IDD10, a key transcription factor in ammonium signaling, was identified to reduce phosphorylation level of S313 residue. Further biochemical analysis using IDD10-GFP transgenic plants and immunoprecipitation assay confirmed that NH4+ supply reduces IDD10 phosphorylation level. Phosphorylation of ammonium transporter 1;1 (AMT1;1) was increased upon NH4+ treatment. Interestingly, phosphorylation of T446, a rice specific residue against Arabidopsis was identified. It was also established that phosphorylation of T452 is conserved with T460 of Arabidopsis AMT1;1. Yeast complementation assay with transformation of phosphomimic forms of AMT1;1 (T446/D and T452/D) into 31019b strain revealed that phosphorylation at T446 and T452 residues abolished AMT1;1 activity, while their plasma membrane localization was not changed. Our analyses show that many proteins were phosphorylated or dephosphorylated by NH4+ that may provide important evidence for studying ammonium uptake and its mediated signaling by which rice growth and development are regulated.
Abstract Soluble N-ethyl-maleimide sensitive factor attachment adaptor protein receptor (SNARE) domain-containing proteins were mainly involved in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNARE in different aspects of plant biology. Among them, Synthaxin-22 (SYP22) a Qa-SNARE has been reported to have a pleiotropic function in plant development including regulation of leaf waving, shoot gravitropism and flowering time. In this study, we identified a new role of SYP22 in regulation of brassinosteroid (BR) signaling, especially in the dark. SYP22 interacts with BR receptor, brassinosteroid insensitive 1 (BRI1), and overexpression of SYP22 enhanced a weak BRI1 mutant bri1-5 phenotype. syp22 mutant exhibits short hypocotyl and it is sensitive to exogenously treated BR while slightly insensitive to BR-biosynthesis inhibitor propiconazole (PCZ) in the dark. Expression levels of BR signaling maker genes ACS5, SAUR15 and IAA19 were slightly higher, while BR6OX2, a BR biosynthesis marker gene, was lower in syp22 compared to the wild-type. In addition, syp22 was sensitive to 2,4-D, a synthetic auxin, in the dark. In conclusion, SYP22 is involved in BR- and auxin-mediated hypocotyl growth inhibition in the dark, which might be via interaction with BR and auxin key regulators to alter their internalization in Arabidopsis.
Abstract Extensive studies have been performed to elucidate the role of brassinosteroids (BRs), an important class of phy-tohormone in plant growth, development, and photomorphogenesis. Different wavelengths of light recognized by photoreceptors play a crucial role in plant development. The role of different photoreceptors in BR signaling has not been analyzed. Here we used photoreceptor single mutants, double mutants and even a quadruple mutant to analyze BR-dependent hypocotyl growth and gene regulation. All the photoreceptor mutants differed from the controls in their response to BR, and hypocotyl elongation as well as BR marker gene regulation were inhibited by application of propiconazole (PCZ), a BR biosynthesis inhibitor. In addition, altered Phytochrome and Cryptochrome expression in brassinosteroid insensitive 1 mutant bri1-5 and brassinazole-resistant 1 dominant mutant bzr1-D indicated that BR negatively regulates photoreceptors in transcriptional levels. This is the first study to investigate the connections between BR and photoreceptors in Arabidopsis.
The objective of this paper is to evaluate the self- healing properties of a commercially-available geosynthetic clay liner (GCL) using flexible-wall permeameter. The GCLs are produced by the same factory, but the contents of bentonite are different. Also the hydraulic conductivities (HC) of GCLs with no defect are different. In this study, specimens were completely saturated under the backpressure of 20 kPa before the test. Permeability tests were performed on GCL specimens with penetrating flaw and also on specimens permeated with distilled water and CaCl2 solutions. The test results were presented and discussed. Experimental results showed that the GCL with penetrating flaw did not exhibit complete self-healing in the case of flaw. After 120 days, the hydraulic conductivity increased by approximately an order of magnitude. In addition, CaCl2 solutions had a significant influence on the hydraulic conductivity. The research findings might be of interest to researchers and engineers who design liners for landfills and other liquid containment facilities
Contamination of soil with heavy metals has become a worldwide environmental problem, and receives great attention. In this study, we aim to investigate soil pollution level affected by an industrial district nearby. The total amount of typical heavy metals in the soils (Hengyang Songmu Industrial Park, Hunan Province, China) was analyzed. In addition, the fraction analysis and laboratory simulation leaching via different pH rainwater was carried out to study the migration and transformation of heavy metals. The main results show that the contents of Cu, Zn, Pb, Cr and Cd in the samples were higher than the soil background values in Hunan Province. The heavy metals forms, analyzed by sequential extraction method, show that the proportion of the unstable form of Cd, Zn and Pb was more than 50%. Igeo values indicate that the heavy metal pollution degree of soil sample #5 at the investigated area is recorded in the order of Cd(6.42), Zn(2.28), Cu(1.82), Pb(1.63), and Cr(0.37). Cu, Zn, Pb, Cr and Cd in this area could pose a potential leaching risk to the environment which may affect the food chain and constitute a threat to human health. It would be necessary to take steps to stabilize and monitor the heavy metals in soil.
Most researchers have explored noise reduction effects based on the transfer matrix method and the boundary element method. However, maximum noise reduction of a plenum within a constrained space, which frequently occurs in engineering problems, has been neglected. Therefore, the optimum design of multi-chamber plenums becomes essential. In this paper, two kinds of multi-chamber plenums (Case I: a two-chamber plenum that is partitioned with a centre-opening baffle; Case II: a three-chamber plenum that is partitioned with two centre-opening baffles) within a fixed space are assessed. In order to speed up the assessment of optimal plenums hybridized with multiple partitioned baffles, a simplified objective function (OBJ) is established by linking the boundary element model (BEM, developed using SYSNOISE) with a polynomial neural network fit with a series of real data – input design data (baffle dimensions) and output data approximated by BEM data in advance. To assess optimal plenums, a genetic algorithm (GA) is applied. The results reveal that the maximum value of the transmission loss (TL) can be improved at the desired frequencies. Consequently, the algorithm proposed in this study can provide an efficient way to develop optimal multi-chamber plenums for industry.