We used the Dpph method to assess in vitro the antiradical activity of extracts from the roots, leaves and fruits of six Rumex L. (dock) species. Data from preliminary screening indicated that all the tested extracts showed antioxidant properties. The degree of antiradical activity depended upon the plant part. Fruit extracts from R. hydrolapathum Huds., R. obtusifolius L. and R. confertus Willd. showed stronger antiradical properties than the other tested material. We also determined tannin content levels in the extracts and their correlation with antioxidant activity.
The experiment on Zea mays L. cv. Landmark (F1) plants was performed in a greenhouse with UV-B (305–315 nm). The pots with plants were divided into four groups: the first and the second groups were grown, respectively, at low (1.0 kJ m-2 d-1) and high (3.0 kJ m-2 d-1) biological effective dose of UV-B radiation. Half of the pots of each group were sprayed with 0.1% solution of Asahi SL (the third and fourth groups). The intensity of photosynthesis and transpiration, chlorophyll fluorescence, the content of UV-absorbing compounds and radical scavenging activity were measured using DPPH after four and six weeks of UV-B radiation. After six weeks of irradiation with a higher UV-B dose both flavonoid content and antioxidant activity increased by 112% and by 44%, respectively, compared to the plants grown at the lower dose. The plants treated with Asahi SL and exposed to the high dose of UV-B had the content of flavonoids 80% higher than the control ones. Asahi SL decreased scavenging activity in both groups of plants by 17% and 32%, respectively, in comparison with the untreated plants. The intensity of net photosynthesis, the transpiration rate and chlorophyll fluorescence parameters (Fv/Fo, ETR, Rfd) did not differ in most of variants.
We used DPPH scavenging assays to study the antioxidant activity of three native Polish species of blackberry leaves (Rubus kuleszae Ziel., R. fabrimontanus (Sprib.) Sprib. and R. capitulatus Utsch.). All the studied extracts (methanolic, water, methanolic-water) showed high DPPH free radical scavenging activity (IC50 450.0-186.0 μg/ml). The most effective of the studied species was Rubus kuleszae. Total content of phenolic compounds (70.50-136.04 mg GAE/g) and phenolic acids (14.70-38.26 mg CAE/g) was determined spectrophotometrically. Antioxidant activity correlated positively with total content of phenolic compounds and phenolic acids.