Insulin receptor substrate 2 (IRS-2) modulates the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, which controls the suppression of gluconeogenic genes; IRS-2 is also a critical node of insulin signaling. Because of the high homology between pig and human IRS-2, we investigated the expression pattern and function of porcine IRS-2. QPCR and immunoblotting were used to detect the IRS-2 expression level in different tissues. There were high IRS-2 levels in the cerebral cortex, hypothalamus, and cerebellum in the central nervous system. In peripheral tissues, IRS-2 was expressed at relatively high levels in the liver. Immunohistochemistry analysis revealed that IRS-2 was mainly distributed in the hypothalamus and cerebral cortex. Furthermore, IRS-2 knockdown porcine hepatocytes and porcine aortic endothelial cells (PAECs) were generated. The IRS-2 knockdown induced abnormal expression of genes involved in glycolipid metabolism in hepatocytes and reduced the antiatherosclerosis ability in PAECs. In addition, we disrupted IRS-2 in porcine embryonic fibroblasts (PEFs) using the CRISPR/Cas9 genome editing system, before finally generating IRS-2 knockout embryos by somatic cell nuclear transfer (SCNT). Taken together, our results indicate that IRS-2 might be a valuable target to establish diabetes and vascular disease models in the pig.
IR spectroscopy, density and ultrasonic velocity measurements have been carried out for aluminum lithium phosphate glasses with and without MoO3. The observed changes in the FTIR spectra of the glasses were related to the modifier/former role of molybdenum ions. The results revealed that the density increases with increasing MoO<sub>3</sub> content, which was attributed to the increase in the compactness and packing of the glass network. The ultrasonic data were analyzed in terms of creation of new bonds of MoO<sub>3</sub> attached to phosphate units. The new bonds increased the average crosslink density and the number of network bonds per unit volume along with a strengthening of the different modes of vibrations which in its turn increased the ultrasonic velocity, the rigidity and hence the elastic moduli of the glasses.
The paper reports on the photoelectrical performance of the long wavelength infrared (LWIR) HgCdTe high operating temperature (HOT) detector. The detector structure was simulated with commercially available software APSYS by Crosslight Inc. taking into account SRH, Auger and tunnelling currents. A detailed analysis of the detector performance such as dark current, detectivity, time response as a function of device architecture and applied bias is performed, pointing out optimal working conditions.
The paper presents the results of studies on the influence of the 2010 Vistula flood on the humification process in the bottom sediments of the Goczałkowice Reservoir in southern Poland. Due to its location in the vicinity of farmlands, forests and urbanized areas, the Goczałkowice Reservoir is characterized by amplified and intense humification processes within its sediments. The studies were focused on the determining the influence of the flood wave containing organic and inorganic suspensions on these processes. Humic acids were analyzed using two spectroscopic methods: Electron Paramagnetic Resonance (EPR) and Fourier Transform Infrared Spectroscopy (FT-IR). Application of these methods allowed to determine the values of free radicals and of the g-factor, which are indicators of oxidation, aromatization and maturation of humic acids during the humification process, as well as the value of the 1650/1720 ratio, reflecting the dissociation of the COOH group to COO− and the formation of complexes of transitional metals with humic acids during the humification process.
Noise spectroscopy as a highly sensitive method for non-destructive diagnostics of semiconductor devices was applied to solar cells based on crystalline silicon with a view to evaluating the quality and reliability of this solar cell type. The experimental approach was used in a reverse-biased condition where the internal structure of solar cells, as well as pn-junction itself, was electrically stressed and overloaded by a strong electric field. This gave rise to a strong generation of a current noise accompanied by local thermal instabilities, especially in the defect sites. It turned out that local temperature changes could be correlated with generation of flicker noise in a wide frequency range. Furthermore, an electrical breakdown in a nonstable form also occurred in some specific local regions what created micro-plasma noise with a two-level current fluctuation in the form of a Lorentzian-like noise spectrum. The noise research was carried out on both of these phenomena in combination with the spectrally-filtered electroluminescence mapping in the visible/near-infrared spectrum range and the dark lock-in infrared thermography in the far-infrared range. Then the physical origin of the light emission from particular defects was searched by a scanning electron microscope and additionally there was performed an experimental elimination of one specific defect by the focused ion beam milling.
In virtual acoustics or artificial reverberation, impulse responses can be split so that direct and reflected components of the sound field are reproduced via separate loudspeakers. The authors had investigated the perceptual effect of angular separation of those components in commonly used 5.0 and 7.0 multichannel systems, with one and three sound sources respectively (Kleczkowski et al., 2015, J. Audio Eng. Soc. 63, 428-443). In that work, each of the front channels of the 7.0 system was fed with only one sound source. In this work a similar experiment is reported, but with phantom sound sources between the front loud- speakers. The perceptual advantage of separation was found to be more consistent than in the condition of discrete sound sources. The results were analysed both for pooled listeners and in three groups, according to experience. The advantage of separation was the highest in the group of experienced listeners.