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Number of results: 5
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Abstract

The aim of the paper was to study the epizootic situation of babesiosis in the cattle population in eastern Poland and possibly to determine what species of protozoa infects Polish cattle. Blood samples for molecular analysis (real time PCR) were collected from 192 dairy cows from various farms located in eastern Poland. The infection was detected in 10.4% of the samples. All animals were infected with Babesia occultans which sequence of the 18S RNA gene fragment showed a 93.1%, homology with the sequence of B. occultans EU 376017. This is the first report about the detection of B. occultans DNA in asymptomatic cattle in eastern Poland.
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Abstract

Eye fluids (aqueous humour and vitreous humour) may be helpful in estimating ante-mortem blood levels, since some parameters measured in these fluids have proved to be stable or to change in a predictable way after death. This would help in diagnosing the cause of death in some diseases or to evaluate ante-mortem blood levels in certain animals not easy to handle or with difficult access. In order to establish reference values of some parameters in blood and eye fluids (aqueous humour and vitreous humour), as well as the possible correlation among these three different fluids, various minerals and electrolytes (Ca, P, Mg, K, Na, Fe, Cr, Co, Ni, Cu, Zn, Se and Mo) were measured in 15 four to five year-old Lidia bulls, all dying after a period of significant stress and major exertion. Plasmatic values of Mg and P were much greater than reported in the literature. In general, mineral plasmatic values were greater than those found in ocular fluids (aqueous and vitreous), while Na, K and Cr were similar in the three fluids. We have verified the existence of correlations in P, Co and Mo among the three fluids measured, and between Se of plasma and vitreous humour. But the most marked correlations were observed in Mo (plasma -aqueous humour, r = 0.893, plasma-vitreous humour, r = 0.945, HA -HV, r = 0.849), in P (plasma-vitreous humour, r = 0.726) and in Co (plasma-vitreous humour, r = 879).
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Abstract

The aim of this study was to implement ADM1xp model to simulate behavior of anaerobic co-digestion of maize silage and cattle manure. The accuracy of ADM1xp has been assessed against experimental data of anaerobic digestion, performed at OLR = 2.1 gVS dm-3·d-1 and HRT = 45d. Due to the high number of parameters in ADM1xp, it was necessary to develop a customized procedure limiting the range of parameters to be estimated. The best fitting of experimental to simulated data was obtained after verification of 9 among 105 stoichiometric and kinetic parameters. The values of objective function (Jc) ranged between 0.003 (for valerate) and 211 (for biogas production).
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Abstract

This paper presents the results of fractionation of particulate and soluble organic matter in a mixture of maize silage and cattle manure (49:51% volatile solids) that was used as a feedstock for anaerobic digestion. The extended Weender’s analysis was adapted to measure raw protein, raw lipids, fraction of carbohydrates (including starch, cellulose, hemicelluloses) and lignin. The content of individual fractions in composite, Xc (as kg COD kg-1 COD) was: 0.111 proteins, 0.048 lipids, 0.500 carbohydrates and 0.341 inerts. The biodegradability of Xc was 68%. Based on material balance, the carbon concentration in Xc was 0.0326 kmol C kg-1 COD, whereas nitrogen concentration 0.0018 kmol N kg-1 COD. The estimated pH of the feedstock based on acid-base equilibrium corresponded to the actual value (pH 7.14).
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Abstract

The aim of the present study was to investigate whether the anterior chamber constitutes part of the normal migratory pathway of CD4+ and CD8+ lymphocytes in cattle and swine. The cells obtained from aqueous humor of cows and pigs were stained for CD4 and CD8 receptors, and subsequently analyzed with flow cytometry. The mean percentage of CD4+CD8-, CD4-CD8+ and CD4+CD8+ cells within the total lymphocyte population of the bovine anterior chamber was, respectively, 17.88, 12.64 and 27.26%. In turn, the mean values of these parameters in pigs were 1.77, 38.48 and 17.45, respectively. Among bovine and porcine CD4+CD8+ cells prevalent were those displaying CD4lowCD8low and CD4lowCD8high phenotypes, respectively. The results suggest that the anterior chamber in cattle and swine is an element in the normal migratory pathway of CD4+, CD8+ and CD4+CD8+ cells. Furthermore, the contribution of these subsets in the anterior chamber lymphocyte population can differ considerably between animal species.
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