Notothenia coriiceps, N.rossii and Lepidonotothen nudifrons were sampled from Admiralty Bay from March to November 1997 and from January to February 1999. The ratio of N.coriiceps to N.rossii individuals in catches regularly increased during 22 years from 0.4 in 1977 to 9.5 in 1999. Oving to its reproductive strategy and avoidance of shallow waters N.rossii may be more vulnerable to overfishing. The condition factor was the most stable (CV 6 -10%), variations in the of hepatosomatic index and index of stomach fullness were intermediate (21 -38%and 40 -43%,respectively), and the gonadosomatic index was the most variable (65 -100%). Lack of seasonality in two somatic indices (condition factor and index of stomach fullness) in N.coriiceps adults is a direct effect of high food availability and unlimited food detection all year round. In contrast, the seasonality of a reproductive index (gonadosomatic index) of these fish is a response to the highly seasonal trophic conditions for pelagic larvae.
An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L-1 BA and 0.05 mg L-1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L-1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species