At the ice edge krill undergoes diurnal migrations with the period of 12 hours and amplitude of about 6 meters. The mean depth of krill occurrence is 41 m, shallower then for open waters. In our opinion these migration parameters are characteristic of juvenile adolescent krill dominating at the ice edge.
The method of target strength measurement adopted for the krill's target strength determination is proposed. The relation between the length of krill's individual and its target strength, obtained at the laboratory conditions, is presented.
During SIBEX the acoustically evaluated amount of krill in the Bransfield Strait and Drake Passage was very low with the mean density 3.24 individuals/m2 and 4.29 individuals/m2 accordingly. Any substantial quantities of krill were found North-West from the Elephant Island and North from the King George Island, where the density of krill exceeded 1000 individuals/m2 (about 100 t/nM2]). The total biomass was estimated at 70590 ton in the Bransfield Strait and at 122470 ton in the Drake Passage, which was many times less than during FIBEX 81, especially in the Bransfield Strait.
Using a thin layer chromatography the content and composition of krill lipids was examined in different sex and maturity stages. The content of lipids decreased in the following sequence: immature males — females with eggs — juvenile specimens — spent females — mature males. In females the differences concerned mainly phospholipids and waxes, in males — triacylglicerols; this fact proves the different utilisation of lipids for reproduction in both sexes.
A composition of lipids of some Antarctic Crustacea (Euphausia superba. E. triacantha. Thysanoessa macrura and Mysidacea gen. sp. indet.) caught in the Admiralty Bay (South Shetlands) was compared. Lipids of E. superba differed in low content of waxes that evidences for different management of lipids than in other examined Crustacea.
The photo-oxidability of lipids taken from 32 samples of krill from different dates of catch has been examined for photooxidation. Relations were indicated between the rate of accumulation of peroxides in the process of lipids, exposure and content of lipids in krill, its iodine value and amount number of carotenoids.
On the basis of acoustically registered cross-sections of krill aggregations, regular, irregular and layer forms were distinguished. Regular forms are most frequently observed during spring and in the day time, while irregular forms are most frequent during summer and night hours. The density histograms made for two hour intervals clearly show the day-night difference, but the seasonal (spring, summer) difference is less pronounced. Mean density of swarm is lowest during the night and reaches a maximum in early morning hours. The mean volume backscattering strength values (Sv) for spring and summer are nearly identical. We suggest that regular forms correspond to foraging swarms and irregular forms to feeding swarms as described by Hamner (1984).
An attempt at assessing the correlation between the content of fluoride in the Antarctic krill from various fishery and its.biological condition was carried out Fluoride was determined with the Dolan method, which was modified by the present authors. No statistically significant correlation was found between the degree of sexual maturity and fluoride content. There was no decisive statistical relation between the body weight and body length of individuals and the content of fluoride in the Antarctic krill.
Concentration of Zn, Cu, Cd, Pb and Co have been determined in Antarctic water (South Shetland Islands) and in krill exoskeletons with the help of atomic absorption spectrophotometry. Concentrations of these metals both in sea-water and in krill exoskeleton are in order Zn > Cu > Cd > Ni > Pb > Co. Comparing concentrations of these metals in sea-water to their concentrations in krill exoskeleton, the factors have been calculated giving a list of metals in the order of krill chitin ability, which is Ni > Cu > Zn > Cd > Pb > Co accumulation. The highest accumulation factors for Ni and Cu point out to the special role played by these metals in krill life.
The amount and composition of lipids m some Antarctic animals were studied. The material consisted of crustaceans (Euphausia superba, Paramoera sp., Orchomene sp.), tunicates (Salpa thompsoni) and vertebrates (Notothenia rossi marmorata and Hydrurga leptonyx). The author's data are discussed on the background of available literature information.
In the investigated area the overall abundance of krill was small and was increasing with the distance from ice. However, with the data available, it was not possible to decide whether this increase was related to the ice border or was a part of a larger scale phenomenon. The depth distributions as well as the mean values of krill depth were similar to those of open water both in this study and reported in literature.
The highest infestation by phoronts (resting stages) of Apostoma ciliates forms 1, 2, is restricted to the 3-th and 4-th pairs of E. superba thoracic limbs. They occur mostly on meropodites of endopodite and plumose setae of exopodite. The trophonts (trophic stage) of those Apostoma are present in large numbers in krill's tissue. The life cycle of those histophagous Apostoma include also free-living stage - tomit. Swarm formed by krill seems to be a reason for the common and extensive infestation by protozoans.
The presence of Euphausia superba, E. crystallorophias and Thysanoessa macrura was observed in Admiralty Bay (King George Island, South Shetland Islands) and the size of individuals of particular species are diverse and varying during the summer season. E. superba population is older and specimens larger than in analogous season in 1979. The maximum number of females with eggs was noted in the first half of January 1980, i.e. earlier than in 1979. In E, crystallorophias population the presence of females with eggs was observed in the second half of December 1979.
On the basis of measurements of the depth of occurrence of 11000 krill aggregations and the biological analyses of these animals and measurements of some environmental factors the diurnal vertical distribution of aggregations is presented against the background of various environmental conditions. Vertical distribution of aggregations is closely related to the feeding rhythm of krill. Active vertical migrations have been recorded at civil twilight. The increasing and decreasing rate of aggregations in those periods is described.
Recording of krill swarms and the observations of the state of the sea and the force of wind were conducted on the M/T "Gemini" from 6 to 26 February, 1978, eastwards of the South Orkneys Archipelago. It has been found that a heavy sea and strong winds disperse krill swarms. At night krill swarms occur much more frequently than during the day.
This bibliography presents a list of 169 papers of Polish authors, treating on the Antarctic zooplankton. The majority of these papers (67%) concern Antarctic krill (Euphausia superba Dana), mainly its biology, ecology and physiology. Quite numerous papers by Polish authors concerning the biochemistry of krill as well as its fishing technique and food - processing are here omitted.
Results of hydroacoustic investigations of krill biomass carried out in the South Shetland Island region between October 1986 and January 1987 are presented. A considerable difference in the krill biomass between Antarctic spring and summer was recorded. Initially observations were conducted close to Elephant Island, in the period just after the retreated of compact ice cover. Krill then aggregated only in swarms, the density of which frequently exceeded 100 t nM-2 . In the region of Polygon I (30—31 October 1986) the total estimated biomass was 26899 t, in the region of Polygon II (6—10 November 1986) it was 25827 t. Investigations were repeated in January 1987 obtaining 112372 t in the Bransfield Strait and 390309 t in the region of Elephant Island. The results are presented in tables and maps.
On the basis of hydroacoustic observations it is shown that migrations of krill during spring are stronger than during summer. Migrations of krill are described by the function: H(t) = A + Bcos((2ᴨt/T + φ ) + C c o s ( 2 ᴨt/T + φ ), where: H is depth of the mass center of krill biomass, A — mean depth of krill occurrence, В — amplitude of migrations with period T! = 24 h, С — amplitude of migrations with period T2 = 12 h, (φ1, φ2 — phases of migration process with T, = 24 and T2 = 12 hours. Parameters of the equation are the following: spring — A = 62.2 m, В = 19.5 m, С = 4.6 m, φ1 = 0.1 h, φ2 = 0 . 1 5 h; summer — A = 75.8 m, В = 0.5 m, С = 3.6 m, φ1 = 1.8 h, φ2 = 6.4 h.
In the investigated area krill occured in low abundance. It was recorded mainly above the shelf and above the continental slope close to the Palmer Archipelago and near the northern shores of Elephant Island. In the central part of the Bransfield Strait E. superba was caught in especially small quantities. In general krill of small size occurred, the size decreasing from the west to the east. Mature krill was dominan in the western part of the investigated area, whereas juveniles in the eastern part. Gravid females were caught very rarely.
Lipolytic activity was assayed in samples of Antarctic krill frozen in different conditions and in its liquid digesta with synthetic (tributylglycerol, esters of 2-naphtol and fatty acids C3, C9 , C14 and C18 ) and natural (olive oil) substrates. It was testified that the lipolytic activity is several-fold higher in the crustaceans with high food intake than in those with an empty digestive tract. Krill lipases show higher activity against esters of unsaturated fatty acids that against analogous derivatives of saturated ones and 10-fold higher affinity tributylglycerol (Km = 1.12 mM). Their maximal activity is at pH 6.4 and 37°C. E. superba lipases preserve total activity up to 35°C for 45 minutes, and are completely inactivated at 55°C for 5 minutes. Prevailing part of lipolytic activity is present in krill cephalothorax, however, extracts from krill abdomen also display a marked activity. Krill lipases are probably resistant to an attack of crustacean's proteinases.
The length of crystalline cones (cc) is proportional to krill body length and this proportion can be described by the equation L cc = L krill x 1.679 + 52.032 ( cc — μm; L krill - mm). By measuring cc one can determine the size of krill with the precision of 2—3 mm. The structure of crystalline cones is not crystal, and the elemental composition includes much of S and Ca. Crystalline cones are often found in the stomach and feces of animals feeding on krill.
Distribution and population age structure of krill Euphausia superba larvae is presented for four consequent years on the basis of the summer materials from 1976 to 1979. An approximate rate of growth of krill larvae was calculated on the basis of the above observations and the literature data on the occurrence of particular larval stages. This allowed to determine the main period of krill breeding in particular years. Intense breeding of krill took place from January to middle of March in summers 1976/1977 and 1977/1978, but much earlier — mid November to mid January in 1975/1976. In summer of 1978/1979 the breeding of krill was poor till March.
Polish exploration and exploitation of marine resources of Antarctic waters date back to the reconnaissance cruise of the Sea Fisheries Institutes (SFI) r/v Profesor Siedlecki in 1974. Since 1975, a co-operation between the Institute of Ecology, Polish Academy of Sciences (PAS) at Dziekanów Leśny and SFI in Gdynia with participation of the University of Agriculture in Szczecin, Faculty Marine Fisheries and Food Technology (UA) was established. Fishing fleets of the Polish Deep-Sea Fisheries Companies Odra, Dalmor and Gryf, since 1976 were operating in the Atlantic sector of Antarctic waters, south of the convergence.
Antarctic krill carbohydrate content was followed during 1983—84 Eighth Polish Antarctic Expedition. The Admiralty Bay (King George Island) was th area of study. The following average values of three estimated fractions were obtained: 3.77 +- 1.51%, 0.47 +- 0.34% and 3.30 +- 1.33% for total, TCA-soluble and TCA-insoluble carbohydrates, respectively. Percentage contribution of the estimated fractions to dry weight varied seasonally (1.48—7.41%, 0.15—1.83%, and 1.28—6.28%, respectively). The carbohydrate content showed a clearcut cycle of changes over the calender year, with a minimum in autumn-winter and a maximum in spring-summer.
ll was proved that the activity of basic proteinases (pH 8.3) and acid proteinases (pH 4.0) of the Antarctic krill increases exponentially in spring-summer season (September-December); the activity of the first ones is 6 times higher and increases more rapidly. The positive relation between the proteolytic activity and the degree of gut filling of krill was also evidenced. The lack of high activity of acid proteinases in early spring does not support the suggestions of Ikeda and Dixon (1982) that during Antarctic winter krill takes energy from the autoproteolysis of own body proteins.